Part:BBa_K4256205:Design

2-phenylethanol synthesis pathway

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2490
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

To reduce the metabolic stress of cells and avoid the problem of plasmid incompatibility when designing this pathway, we chose to use polycistron for expression. Due to the pandemic, we did not have enough experimental time to perform experiments on the effect of spacer sequences on transcription efficiency. Thus the spacer sequences between different genes in the polycistronic selected the spacer sequences in the lactose operon.

Source

BcaT： Ornithobacterium rhinotracheale; Aro10 and ADH： Saccharomyces cerevisiae